Agronomy, Vol. 14, Pages 903: Ultrasound-Assisted Alkaline Pretreatment of Biomass to Enhance the Extraction Yield of Valuable Chemicals

Agronomy, Vol. 14, Pages 903: Ultrasound-Assisted Alkaline Pretreatment of Biomass to Enhance the Extraction Yield of Valuable Chemicals

Agronomy doi: 10.3390/agronomy14050903

Authors: Adina I. Gavrila Anamaria Vartolomei Ioan Calinescu Mircea Vinatoru Oana C. Parvulescu Grigore Psenovschi Petre Chipurici Adrian Trifan

As a renewable and sustainable resource, lignocellulosic biomass serves as a crucial raw material for the production of biofuels, biochemicals, and various value-added products. This paper aims to develop and optimize a mild alkaline treatment of sawdust assisted by ultrasound, along with enzymatic hydrolysis of the pretreated material. The alkaline sonochemical pretreatment emerged as the optimal approach to enhance the susceptibility of cellulose to subsequent enzymatic hydrolysis to improve the yield of reducing sugars. A comparative study was performed using various ultrasonic applicators (horn and bath) and conventional assisted alkaline pretreatment. The ultrasonic-assisted pretreatment revealed a higher delignification of 68% (horn) and 57% (bath) compared with conventional pretreatment. Processes were optimized using a statistical analysis based on a 23 factorial design. The ratios between sawdust and alkaline solution (RSL = 0.5–1.5 g/100 mL), US amplitude (A = 20–60%), and working temperature (t = 30–50 °C) were selected as process factors. The optimal operating conditions to maximize the reducing sugar yield (138.15 mg GE/gsubstrate) were found as follows: a solid/liquid ratio of RSL,opt = 1.25 g/100 mL, US amplitude of Aopt = 60%, and pretreatment temperature of topt = 50 °C. The overall outcomes clearly confirmed the intensification of delignification by ultrasound-assisted alkaline pretreatment.