Fermentation, Vol. 10, Pages 217: Biohydrogen, Volatile Fatty Acids, and Biomethane from Mezcal Vinasses—A Dark Fermentation Process Evaluation

Fermentation, Vol. 10, Pages 217: Biohydrogen, Volatile Fatty Acids, and Biomethane from Mezcal Vinasses—A Dark Fermentation Process Evaluation

Fermentation doi: 10.3390/fermentation10040217

Authors: Sergio A. Díaz-Barajas Iván Moreno-Andrade Edson B. Estrada-Arriaga Liliana García-Sánchez Marco A. Garzón-Zúñiga

Mezcal is a drink made in Mexico, the production of which generates vinasses with a high content of organic matter (OM) that is not utilized. However, these residues have the potential to be drawn upon in dark fermentation (DF) processes to obtain biogas rich in biohydrogen, biomethane, and volatile fatty acids (VFAs) with the potential to become biofuels. In the present work, the effect of reaction time (RT) and organic load (OL) was assessed based on the efficiency of removing OM, the production of VFAs, and the generation and composition of biogas in a process of DF fed with mezcal vinasses. The results show that increasing the RT and decreasing the OL increases COD removal but decreases biohydrogen production. The maximum production of H2 (64 ± 21 NmL H2/Lreactor) was obtained with the lowest RT (1 d) and the highest OL (13.5 gCODm3d−1), while the highest accumulation of VFAs (2007 ± 327 mg VFA/L) was obtained with an RT of 3 d. It was determined that RT and OL are key parameters in DF processes for biohydrogen and VFA production.