Molecules, Vol. 28, Pages 2041: Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells

Molecules, Vol. 28, Pages 2041: Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells

Molecules doi: 10.3390/molecules28052041

Authors: Sultan Alhayyani Abdullah Akhdhar Amer H. Asseri Abdelhafeez M. A. Mohammed Mostafa A. Hussien L. Selva Roselin Salman Hosawi Fahad AlAbbasi Khadijah H. Alharbi Roua S. Baty Abdulaziz A. Kalantan Ehab M. M. Ali

Medicinal plants provide a wide range of active compounds that can be exploited to create novel medicines with minimal side effects. The current study aimed to identify the anticancer properties of Juniperus procera (J. procera) leaves. Here, we demonstrate that J. procera leaves’ methanolic extract suppresses cancer cells in colon (HCT116), liver (HepG2), breast (MCF-7), and erythroid (JK-1) cell lines. By applying GC/MS, we were able to determine the components of the J. procera extract that might contribute to cytotoxicity. Molecular docking modules were created that used active components against cyclin-dependent kinase 5 (Cdk5) in colon cancer, aromatase cytochrome P450 in the breast cancer receptor protein, the -N terminal domain in the erythroid cancer receptor of the erythroid spectrin, and topoisomerase in liver cancer. The results demonstrate that, out of the 12 bioactive compounds generated by GC/MS analysis, the active ingredient 2-imino-6-nitro-2H-1-benzopyran-3-carbothiamide proved to be the best-docked chemical with the chosen proteins impacted by DNA conformational changes, cell membrane integrity, and proliferation in molecular docking studies. Notably, we uncovered the capacity of J. procera to induce apoptosis and inhibit cell growth in the HCT116 cell line. Collectively, our data propose that J. procera leaves’ methanolic extract has an anticancer role with the potential to guide future mechanistic studies.